Destined to set the standard, the first book dealing exclusively with this revolutionary and novel imaging technology serves as an easy-to-understand introduction while offering numerous tips and tricks. Adopting a practical approach, the authors who developed this technique provide a comprehensive, hands-on overview of the basics of light sheet fluorescence microscopy, instrumentation, applications, sample preparation, and data analysis. As a reflection of the interdisciplinary nature of the topic, they merge their expertise in physics, biology, and computer science, giving valuable insider tips taken from their work at major manufacturers. The result is in-depth information on hardware and software solutions for a straightforward implementation of LSFM in the lab.