The textbook "Active Ingredients Enhancement by A. rhizogenes in Hyoscyamus muticus" offers a comprehensive study on optimizing the induction of hair roots from the Egyptian henbane plant (Hyoscyamus muticus) using the Agrobacterium rhizogenes strain LBA 9402/12. The study details the methods for infecting leaf explants, the conditions for growth and cultivation, as well as the analysis of the resulting compounds, particularly hyoscyamine and scopolamine. The results demonstrate significant differences in the efficiency of hair root induction and the concentrations of active ingredients compared to other plant parts. This book is aimed at professionals and students in the fields of plant biotechnology, pharmacology, and botany who are interested in the application of Agrobacterium in plant research.