Affinity Chromatography: Methods and Protocols, Third Edition offers a comprehensive introduction to the fundamentals of affinity chromatography and provides practical knowledge for developing affinity separation protocols. This specialized book guides readers through modern, advanced protocols, molecular modeling, and the investigation of ligand-target interactions. The chapters are clearly structured and include introductions to the respective topics, a list of required materials and reagents, as well as step-by-step, easily reproducible protocols. Additionally, helpful tips for troubleshooting and avoiding common pitfalls are provided. The book is both authoritative and accessible, aimed at anyone interested in the rapid and quantitative isolation of biomolecules with high purity.