The textbook "Biochemical Assessment of Drugs Active Pharmaceutical Ingredients" offers a comprehensive analysis of active pharmaceutical ingredients (APIs) in medications. It highlights the crucial role that APIs play in combating diseases and examines the quality and safety of drugs that depend on the development of new chemical substances. The study focuses on assessing the API concentrations in local medications as well as the microbiological quality of the water used in the Pakistani pharmaceutical industry. The results indicate that the examined medications, including well-known active ingredients such as mefenamic acid and aspirin, fall within acceptable concentration limits. These findings are significant for the pharmaceutical industry, as they emphasize the need for regular quality checks of APIs and raw materials.