The book "Bioinformatics and Drug Discovery" in its second edition provides a comprehensive and up-to-date overview of the latest advancements in drug research. It covers a variety of topics that are significant for modern drug discovery, including new technologies for target identification, genomic analyses, cheminformatics, protein analyses, as well as network and pathway analysis. Each chapter begins with a detailed introduction that explains both the theoretical foundations and the practical applications of the respective technologies. The second part of the chapters provides detailed procedures for utilizing these technologies and software. The structure of the book follows the established format of the "Methods in Molecular Biology(TM)" series, which offers precise descriptions and implementation guidelines that are crucial for optimal results in the laboratory. This book is aimed at scientists who wish to further their education in the rapidly evolving field of drug research.