Bone Research Protocols, Second Edition, is a comprehensive reference book that addresses the latest advancements in bone biology. Significant discoveries have been made in the past decade that have expanded our understanding of the genetic influences on bone cells and their effects on bone density and strength. This book provides a detailed collection of methods commonly used to study bone biology, with a focus on in vitro techniques. The authors, experts in their field, present protocols for the isolation, cultivation, and functional analysis of all types of bone cells, as well as various imaging techniques, including light microscopy and live cell imaging. Additionally, some key in vivo techniques are covered, such as the analysis of bone resorption and imaging methods using X-rays. The clear structure and step-by-step instructions make it a valuable tool for scientists looking to establish new techniques in their laboratories.