The book "Coronavirus Replication and Reverse Genetics" offers a comprehensive analysis of the molecular biology of coronaviruses, which are the largest RNA viruses with a genome size of 27 to 32 kb. These viruses are not only responsible for significant economic losses in livestock but also impact human health, particularly through emerging strains that can cause severe respiratory diseases. The work discusses the structure of the viral genome, the mechanisms of replication and transcription, as well as the development of tools for reverse genetic studies. These studies are crucial for understanding the interactions between the virus and the host and for exploring the molecular basis of viral pathogenesis. Furthermore, the book provides important information on the development of both classical and recombinant vaccines to combat coronavirus infections.