The "Handbook of Acute Oral Toxicity Testing Method" provides a comprehensive overview of the methods for testing the acute toxicity of chemicals. It aims to gather information about the biological activity of chemicals and gain insights into their mechanisms of action. The tests discussed in the book are crucial for hazard identification and risk management related to the production, handling, and use of chemicals. Special emphasis is placed on the Up and Down Method according to OECD Guideline 425, which is applied in classical LD50 studies using laboratory mice and rats. Additionally, the book includes a research paper on acute oral toxicity studies conducted by the authors.