The "Handbook of Biological Confocal Microscopy" is a comprehensive reference book that addresses the latest developments in biological confocal microscopy. The second edition offers an updated and expanded perspective on advancements in laser technology and fiber optics, as well as an understanding of the mechanisms related to photobleaching and phototoxicity. The authors, who are experts in their field, have compiled their insights and experiences to create a valuable resource for students and professionals alike. The book is intended not only as a reference work but also as teaching material that covers the practical aspects of confocal microscopy. The updated bibliography and consideration of the impact of presentation techniques on results make this work an indispensable companion for anyone involved in biological microscopy.