The book "High Content Screening" offers a comprehensive analysis of the tensions between hypothesis-driven and discovery-driven research approaches in the life sciences. It highlights the development and significance of High Content Screening (HCS) as a platform introduced by Cellomics Inc. in the 1990s. HCS has proven to be crucial for understanding the functions of genes and their products at the cellular level. The book discusses the successes of the Human Genome Project and the necessity of empowering both academic and industrial researchers to explore the complex functions of cells. With a focus on discovering active compounds from large chemical libraries, the relevance of HCS for drug research and development is emphasized. The content is significant for both academic and industrial researchers engaged in the discovery and analysis of biological functions.