The book "Hyperspectral Imaging of Nanoparticle and Single Molecule Detection" by Kyuwan Lee provides a comprehensive investigation into the detection of biomolecules with single-molecule sensitivity. This capability is crucial for understanding dynamic processes in living cells. The work utilizes gold nanoparticles as intercellular sensors, which are particularly suitable due to their nontoxic properties and stability against photobleaching. The development of a hyperspectral imaging system enables the detection of various target biomolecules and demonstrates the application of this technology for intracellular biomolecule detection. The book discusses both dark-field and Raman hyperspectral imaging, highlighting the advantages and specific applications of the dark-field based method, especially regarding the detection of mRNA in cells.