The specialized book "Isolation and Biochemical Characterization of Pseudomonas Aeruginosa" offers a comprehensive analysis of the bacterial species Pseudomonas aeruginosa, which is often responsible for nosocomial infections. It details the role of these bacteria in pus formation, including the characteristic blue and green coloration of the pus and the tissue damage they can cause. The book describes the various culture media on which Pseudomonas aeruginosa can be cultivated, as well as the biochemical tests used to characterize these bacteria, such as oxidase and citrate tests. The importance of isolating Pseudomonas aeruginosa from healthy carriers and the environment is emphasized, particularly regarding the risk of transmission to vulnerable patients. The results of the investigation show that all tested strains are glucose and urea negative, which is significant for the identification of these bacteria.