The book "Laser Capture Microdissection: Methods and Protocols, Second Edition" offers a comprehensive introduction to the techniques of laser microdissection, enabling researchers and pathologists to conduct targeted molecular analyses on specific cell types. This second edition includes valuable practical advice from experts in the field to facilitate the execution of tissue-based laser microdissections in their own laboratories. The chapters are structured to provide detailed descriptions of the various laser microdissection technologies available and cover the application of a range of molecular technologies, including PCR, microarrays, and proteomics. The clear organization into introductions, material lists, step-by-step protocols, and troubleshooting tips makes the book an indispensable resource for anyone engaged in molecular biological research.