The textbook "Membrane Protein Structure Determination: Methods and Protocols" offers a comprehensive collection of techniques for determining the structure of membrane proteins, which make up about 40% of all proteins and play a central role in many cellular processes. Despite their importance, only a few structures of these proteins are known. The book brings together contributions from leading experts and is divided into five clear sections. It covers strategies for purifying membrane proteins, methods for obtaining three-dimensional crystals and their structure determination through X-ray diffraction, as well as the use of electron microscopy for structural analysis. Additionally, it presents recent advances in nuclear magnetic resonance (NMR) and molecular modeling strategies that serve both for structure determination and for understanding dynamic molecular mechanisms. The chapters are written in the established format of the "Methods in Molecular Biology" series and include introductions, material lists, step-by-step protocols, and tips for avoiding errors.