Molecular Chaperones: Methods and Protocols is a specialized book that addresses the crucial role of molecular chaperones in protein folding and their significance for the health of organisms. The book provides a comprehensive overview of various methods for studying these mechanisms, including the functioning of heat shock proteins and heat shock transcription factors. The chapters are written in the established format of the Methods in Molecular Biology series and include introductions to the respective topics, lists of required materials and reagents, as well as step-by-step, easily reproducible laboratory protocols. Additionally, helpful tips for troubleshooting and avoiding common pitfalls are provided. This book is aimed at scientists interested in the biological functions of chaperones and their impact on human health and diseases.