The book "Purification and characterization of Amidase from Bacillus sp. APB-6" provides a comprehensive introduction to the fundamental methods for the purification and characterization of the amidase enzyme isolated from Bacillus sp. APB-6. It is aimed at readers who wish to develop a basic understanding of enzyme purification and properties. The author, Sudha Batta, uses clear and accessible language to explain complex concepts. The book includes detailed, step-by-step instructions for enzyme purification as well as for the characterization of the purified enzyme. Additionally, it covers techniques for amino acid sequencing and post-synthetic modifications of the enzyme, made possible through protein engineering techniques.