The book "siRNA Design: Methods and Protocols" offers a comprehensive and detailed guide to the application of RNA interference (RNAi) for gene silencing. This methodology has revolutionized biological research and opened new possibilities in therapeutic development. The work focuses on the design of small interfering RNAs (siRNA), which are considered the most common strategy for implementing RNAi. It includes precise guidelines for selecting siRNA target sequences as well as for the strategic integration of chemical and structural modifications. The provided protocols enable the utilization of endogenous cellular mechanisms to produce siRNA from optimized precursor molecules such as short hairpin RNA (shRNA) and artificial microRNAs (amiRNA). Additionally, specific applications such as immunostimulatory siRNA and strategies for simultaneous targeting of multiple siRNAs are addressed. The book is part of the renowned series "Methods in Molecular Biology(TM)" and is aimed at researchers, educators, clinicians, and professionals in biotechnology.