The BAM Complex: Methods and Protocols is a comprehensive reference book that focuses on the experimental protocols for studying the biogenesis of β-barrel outer membrane proteins in gram-negative bacteria. This work provides a detailed guide to the methods necessary for investigating the function of the BAM complex. It addresses the role of individual components, their expression and purification, as well as crystallization and structure determination. Readers gain valuable insights into the assembly of Bam components into a functional complex. The chapters are clearly structured and include introductions to the respective topics, a list of required materials and reagents, and step-by-step, reproducible laboratory protocols. Additionally, helpful tips for troubleshooting and avoiding common pitfalls are provided. This book is an essential reference for anyone working with the BAM complex.