The book "The Tumor Microenvironment" offers a comprehensive analysis of the various components of the tumor microenvironment and their interactions with tumor cells. It covers both established and novel in vitro and in vivo protocols used to study carcinogenesis. The authors, who work in renowned laboratories, present a variety of research topics, including cellular and molecular approaches, genetic methods, various omics strategies, and therapeutic approaches aimed at specifically influencing the microenvironment. Additionally, advanced techniques in the fields of tissue engineering and nanotechnology are discussed. Each chapter includes an introduction to the respective topic, a list of required materials and reagents, as well as step-by-step, reproducible laboratory protocols complemented by practical troubleshooting tips. This book is aimed at a broad audience, including basic and clinical scientists, systems biologists, and biological engineers.