The book "Virus-Induced Gene Silencing: Methods and Protocols" provides a comprehensive introduction to the virus-induced gene silencing technique (VIGS), which has established itself as an effective method for targeted downregulation of gene expression in plants. It is aimed at researchers and students in the field of botany and plant sciences, covering both model and non-model plants. The detailed protocols and methods presented in this specialized book enable readers to successfully conduct VIGS experiments and understand the underlying molecular mechanisms. Additionally, innovative approaches for generating VIGS-derived micro-RNAs and for protein overexpression are discussed. The clear structure and step-by-step instructions make it a valuable reference for anyone engaged in functional genomics and the challenges of studying gene function in plants.