The textbook "Zymography" offers a comprehensive examination of the latest techniques and protocols for detecting enzyme activity on electrophoretic gels. It covers various aspects of zymography, including endopeptidase zymography, reverse zymography, in situ zymography, and 2D zymography, as well as special application cases. The chapters are clearly structured and include brief introductions to the respective topics, a list of required materials and reagents, and step-by-step, easily reproducible laboratory protocols. Additionally, helpful tips for troubleshooting and avoiding common pitfalls are provided. This book is a valuable resource for researchers in the fields of biology and medicine, as well as for scientists working in clinical and diagnostic medicine, medical genetics, agricultural entomology, genetic monitoring of environmental pollution, and forensic science.