The book "Advances in Mass Data Analysis of Signals and Images in Medicine, Biotechnology and Chemistry" offers a comprehensive examination of the automatic analysis of images and signals in the fields of medicine, biotechnology, and chemistry. It highlights the challenges and requirements associated with the mass analysis of signal information generated by various sensors such as microscopes and spectrometers. Given the growing importance of these technologies in various application areas, including environmental analysis and industry, the need for new methods and system architectures becomes evident. The content is based on the results of the International Conference on Mass Analysis of Images and Signals, which brings together researchers, practitioners, and industry professionals to discuss current developments and research findings. The book aims to identify key contributions to automatic image analysis and promote their application in biology, medicine, and chemistry.