"Algorithms in Bioinformatics" is a comprehensive collection of expert contributions documenting the results of the 14th International Workshop on Algorithms in Bioinformatics, WABI 2014. This workshop took place in September 2014 in Wroclaw, Poland, and is part of the prestigious ALGO conference series. The publication includes 26 carefully selected and peer-reviewed papers that emerged from 61 submitted works. The topics covered range from sequence and genome analysis to phylogenetic reconstruction and the analysis of mass spectrometry data. This diversity of topics reflects the current challenges and advancements in bioinformatics and provides valuable insights for researchers and practitioners in the field.