Animal Cell Biotechnology: Methods and Protocols, Third Edition is a comprehensive handbook that covers modern and innovative techniques for establishing mammalian cell lines for the production of biopharmaceuticals. It provides detailed guidance on optimizing critical parameters for cell culture, ranging from the laboratory stage to final production. The book is divided into five parts, reflecting the various phases of the production process. The chapters are structured to provide an introduction to the respective topics, include a list of required materials and reagents, and offer step-by-step, easily reproducible protocols along with troubleshooting tips and advice on avoiding common issues. This third edition is a valuable reference for professionals and students engaged in animal cell biotechnology.