Basic Confocal Microscopy is a comprehensive textbook that focuses on the fundamentals of confocal microscopy. It is aimed at students, technologists, and educators who wish to apply this technique in their research. The book is the result of an intensive five-day course established in 2005 and provides a concise yet thorough introduction to the topic. The authors address key aspects such as fluorescence, sample preparation and labeling, as well as the analog-to-digital conversion of data captured with confocal microscopes. Additionally, practical guidance on operating confocal microscopes is provided to facilitate the application of the technique and optimize the quality of the obtained data. The concluding chapters are dedicated to three-dimensional analysis, ethical considerations in confocal imaging, and useful resources that have been helpful to the authors in their own work.