Biomedical research on laboratory animals is only justifiable when it is based on the current state of knowledge. Comprehensive understanding of the needs of the animals entrusted to you is a prerequisite for species-appropriate care. This standard work provides an overview of all aspects that must be considered for the humane use of laboratory animals. For 18 animal species, you will find detailed information on behavior, housing, breeding, and use in experiments. Additionally, it includes the current animal protection regulations in Germany, Austria, and Switzerland, information on experimental planning, organization, and execution, an overview of professional qualifications and further education, as well as biological baseline data for 35 animal species.