"Gene Delivery to Mammalian Cells" is a comprehensive textbook that focuses on methods for targeted gene transfer in mammalian cells. It is aimed at professionals in the biomedical field and provides detailed guidance on various non-viral techniques used in research and therapy. The authors, experienced researchers in their field, explain a wide range of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, and hydrodynamics. Each protocol includes step-by-step instructions, background information, lists of required equipment and reagents, as well as troubleshooting tips. Introductory chapters provide an overview of the methods presented, their advantages and disadvantages, and their successful application in gene transfer. The book is a valuable resource for scientists seeking reliable and effective methods for gene transfer to achieve their experimental goals.