Hormone Assays in Biological Fluids, Second Edition, provides a comprehensive overview of the measurement of hormones in biological fluids, which is crucial for the diagnosis and monitoring of clinical conditions. Given the exponential increase in hormone requirements in clinical practice, it is essential to develop assays that are accessible to researchers with varying equipment. This specialized book covers both common and newer techniques, such as tandem mass spectrometry, and offers detailed methods for a variety of hormones. The chapters are structured to include an introduction to the respective topics, a list of required materials and reagents, as well as step-by-step, reproducible protocols. Additionally, it provides troubleshooting tips and guidance on avoiding known pitfalls. The authority and accessibility of this book make it a valuable resource for students, technologists, laboratory staff, and researchers seeking a deeper understanding of hormone measurement methods.