The textbook "Host-Pathogen Interactions: Methods and Protocols" offers a comprehensive examination of the interactions between host organisms and pathogens. In recent decades, interest in infectious diseases, once considered largely controlled, has significantly increased. This book brings together insights from leading experts in the field and highlights the critical factors that influence the course of infections and the establishment of diseases or asymptomatic colonization by microorganisms. The detailed, step-by-step laboratory protocols focus on the molecular mechanisms of infections caused by fungi, bacteria, and parasites. The chapters are written in the established format of the "Methods in Molecular Biology" series and provide a clear introduction to the topics, a list of required materials, as well as helpful troubleshooting tips and advice on avoiding common pitfalls. This book is a valuable resource for anyone investigating the factors that contribute to the pathogenicity of microorganisms.