In addition to method development, optimizing existing methods is a central task in the HPLC laboratory. This is a task that must be completed today in increasingly shorter timeframes and in a cost-effective manner. The manual provides a solid resource to better tackle this challenge. Internationally renowned authors such as John W. Dolan, Michael McBrien, Veronika R. Meyer, Uwe D. Neue, Lloyd R. Snyder, and Klaus K. Unger address both the general principles and strategies of optimization as well as the specific aspects of various techniques such as RP-HPLC, NP-HPLC, micro- and nano-HPLC, and coupling techniques like LC-MS. The correct column selection and enantiomer separations are also among the topics covered. The authors provide concrete, practical tips as well as relevant background information. Additionally, they offer insights into optimization practices.