The book "In Vitro Methods in Aquatic Ecotoxicology" offers a comprehensive examination of in vitro approaches for studying the toxicology of pollutants in aquatic environments. It is the first work to specifically address this topic and covers a variety of pollutants, including heavy metals, radionuclides, microorganisms, and estrogen-like compounds. The significance of in vitro methods lies in their ability to develop and validate standardized techniques suitable for substance- and species-specific experiments in controlled environments. These methods enable mechanistic studies without the challenges of individual variations among animals and environmental influences, leading to more precise and reproducible results. The book is aimed at professionals and students dealing with ecotoxicological issues and provides valuable insights into modern research techniques.