"Intracellular Delivery II" is a specialized book that focuses on the latest developments in the field of intracellular drug delivery. It serves as a continuation of the first volume and provides in-depth insights into novel nanocarrier designs as well as their characterization and function. The book highlights the applications of these technologies in drug discovery and treatment. Notably, it features chapters that discuss nanofibers as a significant new application in the biomedical field, particularly concerning wound healing and the treatment of diabetic foot and spinal injuries. Another innovative aspect of the book is the introduction of mathematical methods for the assembly of nanocarriers, which are relevant for both simulations and functional applications.