The book "Membrane Computing" presents the revised and peer-reviewed contributions from the 14th International Conference on Membrane Computing, CMC 2013, which took place in August 2013 in Chisinau, Republic of Moldova. It includes 16 selected papers as well as 6 invited talks, carefully chosen from a total of 26 works presented during the conference. Membrane Computing is an innovative research field within computer science that aims to derive computational models and ideas from the structure and functioning of living cells. The focus is on membrane systems, also known as P-systems, which act as distributed and parallel algebraic models and process multiset objects within a localized framework. Communication between compartments and with the environment plays a crucial role in this context.