The book "Metastasis Research Protocols" offers a comprehensive collection of methods for studying metastasis at the cellular and animal levels. In this second volume, the focus is on living cells and tissues to analyze metastatic behavior both in vitro and in animal models. The protocols presented allow researchers to isolate and genetically modify cell lines with varying metastatic potential. Additionally, various assays are described to investigate specific aspects of the metastatic cascade, including cell migration, matrix degradation, and angiogenesis. These methods are crucial for gaining a better understanding of the role of metastasis in living organisms and for unraveling the underlying biological processes.