This laboratory manual provides precise and reproducible "how-to" instructions for the most important microbiological methods. It has proven to be an indispensable companion for anyone looking to gain initial experience, particularly with bacteria, or seeking practical materials for microbiological training. For the third edition, the text has been revised and supplemented in numerous places. The regulations of the Biochemical Substance Ordinance, rapid tests for Gram staining, and epifluorescence microscopy have been newly included.