NLR Proteins: Methods and Protocols, Second Edition provides a comprehensive foundation for the molecular investigation of NLR function in health and disease. This specialized book addresses NLR as receptors of the innate immune system as well as atypical inflammasomes. It offers detailed insights into biochemical and novel bioluminescence techniques for measuring IL-1b, which serves as an indicator for NLR-based inflammasomes. The chapters are written in the established format of the "Methods in Molecular Biology" series and include introductions to the respective topics, lists of required materials and reagents, as well as step-by-step, easily reproducible laboratory protocols. Additionally, important tips for troubleshooting and avoiding known pitfalls are provided. This book is aimed at both new researchers and experts looking to expand their knowledge.