The book "Pesticide Protocols" offers a comprehensive collection of robust methods for the detection of pesticide compounds or their metabolites. These methods are of great importance for monitoring food, environmental conditions, and biological samples, as well as for studies on exposure through food, water, air, and skin or lungs. The procedures described in the book are easily reproducible and include techniques such as gas and liquid chromatography coupled with mass spectrometry, as well as other classical detection methods. The authors place special emphasis on extraction and purification procedures to develop and optimize automated and miniaturized methods. The detailed protocols provide step-by-step instructions, an introduction to the underlying principles, lists of required equipment and reagents, as well as troubleshooting tips and advice on avoiding known issues. Recent advances in pesticide residue analysis allow for the analysis of many pesticides in a single analytical run.