RNA Folding: Methods and Protocols is a comprehensive reference book that focuses on the formation of RNA structures both in vitro and intracellularly. It provides a collection of experimental protocols aimed at investigating RNA folding pathways and characterizing the structure of RNA folding intermediates at the nucleotide or even atomic level. The techniques presented in the book include both established tools with a long tradition in RNA research and advanced, novel methods. These methods cover multiple disciplines, including molecular biology, biochemistry, biophysics, and computational biology. The chapters are written in the established format of the "Methods in Molecular Biology" series and include introductions to the respective topics, lists of required materials and reagents, as well as step-by-step, easily reproducible laboratory protocols. Additionally, tips for troubleshooting and avoiding known pitfalls are provided. This practical and authoritative work serves as an important reference for researchers looking to gain insights into the secrets of these remarkable macromolecules.