The revised and updated 7th edition of this book provides an overview of established and new methods in protein biochemistry and proteomics. It offers solutions to experimental and strategic dead ends and fosters an intuition for the right experiment at the right time. It covers classic techniques such as column chromatography, HPLC, electrophoresis, blots, ELISA, ligand binding assays, antibody production, solubilization of membrane proteins, glycoprotein analysis, and more. A significant focus is placed on modern methods: mass spectrometry, proteomics, and thermal analysis. The 7th edition includes new techniques for determining interactions between proteins or between proteins and small molecules: DARTS, DRACALA, SPROX, and others. Additionally, you will learn how to use mass spectrometry to analyze binding.