The textbook "Aging Methods and Protocols" offers a comprehensive analysis of biological aging processes and their underlying molecular mechanisms. It highlights the various theories developed over the years to explain the causes of aging and discusses the role of genetic and environmental factors in the development of age-related diseases. Given the demographic changes and increasing life expectancy in the population, the need to understand the impact of aging on society is becoming more urgent. The book is aimed at researchers and professionals dealing with the cellular processes of aging and seeking ways to extend healthspan. It provides valuable insights into current research projects, ranging from cellular to organismal studies, and contributes to a deeper understanding of the complex mechanisms of aging.