"Antiviral Methods and Protocols" is a specialized book from the renowned series "Methods in Molecular Medicine," focusing on the development and validation of methods to combat viral infections. Given the growing interest in discovering effective compounds against chronic and acute viral infections, this book provides a valuable collection of protocols developed by experts in the field. The methods included are not only comprehensively described but also feature unpublished approaches that reflect the dynamics and progress in virological research. Each chapter offers detailed instructions, including a list of required reagents as well as useful tips and potential pitfalls identified during the development of the protocols. This manual is aimed at researchers in universities and industry who are familiar with various biological techniques and wish to quickly set up a new assay system. It encourages interaction between readers and authors, which can lead to valuable collaborations.