This textbook provides an overview of current cell culture techniques, conditions, and applications, specifically focusing on human cell culture. It is based on lectures, seminars, and practical courses in stem cells, tissue engineering, regenerative medicine, and 3D cell culture held at the University of Natural Resources and Life Sciences Vienna and the Gottfried Wilhelm Leibniz University Hannover, complemented by contributions from international experts. Therefore, it delivers important theoretical and practical knowledge in a compact and clear way to advanced graduate students on cell culture techniques and the current status of research. The book is written for master's students and PhD candidates in biotechnology, tissue engineering, and biomedicine working with mammalian, specifically human cells. It will be of interest to doctoral colleges and master's and PhD programs.