This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay while also familiarizing them with the possible sources of error in the assay. It provides detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating key parameters such as sensitivity, specificity, accuracy, and limit of detection. In addition, the book explores the advantages and shortcomings of conventional ELISA, as well as various other aspects.