The "Handbook of ELISPOT" is a comprehensive reference book that focuses exclusively on the ELISPOT assay, an important method in biomedical research. Written by recognized experts in the field, the book provides detailed guidance on the planning, execution, and analysis of ELISPOT experiments. The authors share their extensive experience and present reproducible techniques that cover a wide range of topics, including the use of membrane-supported plates, standardization and validation procedures, as well as cell separation techniques. The book also addresses numerous applications of the ELISPOT assay in various research areas, including animal models and human cells, and offers valuable insights into the quantitative aspects of data analysis. It is an indispensable resource for researchers dealing with immunological questions.