The book "Patch-Clamp Analysis: Advanced Techniques, Second Edition" provides a comprehensive and up-to-date overview of the latest developments in patch-clamp technology, which is crucial for studying the cell membrane. This second edition builds on the findings of the first edition and incorporates three additional years of research results. It covers both traditional methods such as whole-cell and single-channel techniques, as well as newer approaches like perforated patch, rapid drug application, loose patch, and macropatch techniques. The authority of the work is underscored by the introduction of three innovative technical advancements in this constantly evolving research field. The book is particularly aimed at neuroscientists who wish to gain a better understanding of the complex mechanisms of the cell.