The aim of this handy, German-language laboratory manual is to present the standard and specialized applications of PCR in a practical and understandable manner. It is aimed at graduates, doctoral candidates, scientists, and teaching assistants who wish to utilize the possibilities of PCR for their molecular biology or diagnostic questions. In this second edition, the established methods have been updated, and important new applications, such as Next Generation Sequencing and Emulsion PCR, have been added. As automation in molecular biology applications progresses, the latter methods require less manual labor, although a general understanding of the individual steps is still necessary. You will gain this know-how from the present PCR methods book.