Proven in practice, legally compliant, and indispensable for professional hygiene: from hand washing to construction measures. The authors consider all areas of application (patients, personnel, ventilation technology, etc.): hygiene, disinfection, and sterilization in outpatient departments, intensive wards, operating theatres, or physiotherapy. They focus on specialist areas (e.g., pediatrics), functional areas (e.g., endoscopy, dialysis), and central facilities (e.g., pharmacy, kitchen). Legal foundations, organization, epidemiology, and more are taken into account. New topics include infection prevention in naturopathic treatment, bioterrorism, and much more. Specific hygiene plans support practitioners in their work.